| 货号 | AF6517-SP |
| 别名 | DKFZP586H2123; peptidase domain containing associated with muscle regeneration 1; Peptidase domain-containing protein associated with muscle regeneration 1; RAMP; RAMPinactive serine protease PAMR1; regeneration associated muscle protease; Regeneration-associated muscle protease homolog | 全称 | Peptidase Domain Containing Associated with Muscle Regeneration 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PAMR1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25891 (Human); 210622 (Mouse); 311252 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human PAMR1 Leu22-Lys737 Accession # NP_056245 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PAMR1 (Peptidase domain associated with muscle regeneration 1; also RAMP/ regeneration-associated muscle protease homolog) is a secreted, 80 kDa (predicted) member of the peptidase S1 family of enzymes. It is expressed by skeletal muscle, and appears to mediate skeletal muscle regeneration. Due to the lack of a catalytic serine at amino acid (aa) 672, PAMR1 demonstrates no enzyme activity. Mature PAMR1 is 716 aa in length (aa 22-737). It contains a Cys-rich segment (aa 42-94), one CUB domain (aa 128-234), an EGF-like region (aa 235-272), two SUSHI domains (aa 278-444) and a nonfunctional peptidase S1 domain (aa 445-737). There is one 17 aa deletion after Glu276. Over aa 21-737, human PAMR1 shares 88% aa identity with mouse PAMR1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PAMR1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
