Human TROP-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TROP-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF650-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF650-SP
别名	Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2GA7331; tumor-associated calcium signal transducer 2
全称	Tumor-associated Calcium Signal Transducer 2
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4070 (Human); 56753 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors. Authors: Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D Clin Cancer Res, 2015;21(17):3870-8. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified Trop-2 is a determinant of breast cancer survival. Authors: Ambrogi, Federico, Fornili, Marco, Boracchi, Patrizia, Trerotola, Marco, Relli, Valeria, Simeone, Pasquale, La Sorda, Rossana, Lattanzio, Rossano, Querzoli, Patrizia, Pedriali, Massimo, Piantelli, Mauro, Biganzoli, Elia, Alberti, Saverio PLoS ONE, 2014;9(5):e96993. Species: Human Sample Type: Whole Cells Application: Flow Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis. Authors: Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L Oncol Lett, 2014;8(4):1670-1674. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Upregulation of Trop-2 quantitatively stimulates human cancer growth. Authors: Trerotola, M, Cantanelli, P, Guerra, E, Tripaldi, R, Aloisi, A L, Bonasera, V, Lattanzio, R, de Lange, R, Weidle, U H, Piantelli, M, Alberti, S Oncogene, 2013;32(2):222-33. Species: Human Sample Type: whole cells Application: ICC Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells. Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H J. Invest. Dermatol., 2011;131(10):2049-57. Species: Human Sample Type: Whole Cells Application: Flow Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody. Authors: Raji R, Guzzo F, Carrara L J. Exp. Clin. Cancer Res., 2011;30(0):106. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy. Authors: Nakatsukasa M, Kawasaki S, Yamasaki K Am. J. Pathol., 2010;177(3):1344-55. Species: Human Sample Type: Cell Lysates Application: IP Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Authors: Wang J, Day R, Dong Y, Weintraub SJ, Michel L Mol. Cancer Ther., 2008;7(2):280-5. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TROP-2 Thr88-Thr274 Accession # P09758
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507. Fornaro, M. et al. (1995) Int. J. Cancer 62:610. Ripani, E. et al. (1998) Int. J. Cancer 76:671. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

参考图片

Detection of Human TROP‑2 by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP‑2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

TROP‑2 in Human Brain. TROP‑2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.