Human Neurocan Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Neurocan Affinity Purified Polyclonal Ab (25 UG)

货号： AF6508-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6508-SP
别名	chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; neurocan
反应种属	Human
应用	Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Neurocan in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Neurocan is observed.
使用方法	Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1463 (Human); 13004 (Mouse); 58982 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Neurocan Glu23-Cys1321 Accession # O14594
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C‑type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C‑terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6-8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10-15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842. Prange, C.K. et al. (1998) Gene 221:199. Rauch, U. et al. (1991) J. Biol. Chem. 266:14785. Matsui, F. et al. (1994) Neurochem. Int. 25:425. Rauch, U. et al. (1992) J. Biol. Chem. 267:19536. Asher, R.A. et al. (2000) J. Neurosci. 20:2427. Matsui, F. et al. (1998) Brain Res. 790:45. Abaskharoun, M. et al. (2010) Brain Res. 1327:6. Shen, L.H. et al. (2008) Glia 56:1747. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669. Sango, K. et al. (2003) Exp. Neurol. 182:1. Akita, K. et al. (2004) Biochem. J. 383:129. Grumet, M. et al. (1994) J. Biol. Chem. 269:12142. Milev, P. et al. (1996) J. Biol. Chem. 271:15716. Rauch, U. et al. (2004) Matrix Biol. 22:629.

参考图片

Neurocan in Human Brain. Neurocan was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6508) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.