Human/Mouse E-Cadherin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse E-Cadherin Affinity Purified Polyclonal Ab (25 UG)

货号： AF648-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF648-SP
别名	Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; E-cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human E-Cadherin in direct ELISAs and Western blots.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	999 (Human); 12550 (Mouse); 83502 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level. Authors: Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, Zhao S PLoS Genet, 2015;11(6):e1005277. Species: Canine Sample Type: Whole Cells Application: IHC Paraffin-embedded Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer. Authors: Liu D, Xiong H, Ellis A, Northrup N, Rodriguez C, ORegan R, Dalton S, Zhao S Cancer Res, 2014;74(18):5045-56. Species: Canine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway. Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P PLoS ONE, 2012;7(2):e31624. Species: Human Sample Type: Whole Cells Application: ICC Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells. Authors: Li H, Wang H, Wang F, Gu Q, Xu X PLoS ONE, 2011;6(8):e23322. Species: Human Sample Type: Cell Lysates Application: WB Tissue engineering of oral dysplasia. Authors: Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M J. Pathol., 2008;215(3):280-9. Species: Human Sample Type: Whole Cells Application: ICC Downregulation of Rap1GAP contributes to Ras transformation. Authors: Tsygankova OM, Prendergast GV, Puttaswamy K, Wang Y, Feldman MD, Wang H, Brose MS, Meinkoth JL Mol. Cell. Biol., 2007;27(19):6647-58. Species: Human Sample Type: Cell Lysates Application: WB Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Authors: Tamai R, Asai Y, Ogawa T Infect. Immun., 2005;73(10):6290-8. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human E-Cadherin Asp155-Ile707 Accession # P12830
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.
运输条件	Blue Ice
存放说明	4℃
参考文献	Bussemakers, M.J.G.et al. (1993) Mol. Biol. Reports 17:123. Overduin, M.et al. (1995) Science 267:386. Takeichi, M. (1991) Science 251:1451.

参考图片

Detection of Human and Mouse
E‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of E‑Cadherin in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Goat Anti-Human/Mouse
E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

E‑Cadherin in A549 Human Cell Line. E‑Cadherin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

E‑Cadherin in Human Epidermoid Carcinoma Cells. E‑Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human and Mouse E‑Cadherin by Simple Western^TM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.