| 货号 | AF6486-SP |
| 别名 | EC 2.4.2.1; FLJ94043; FLJ97288; Inosine phosphorylase; MGC117396; MGC125915; MGC125916; NPFLJ97312; nucleoside phosphorylase; PRO1837; PUNP; purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(50 µg/mL) |
| 目标/特异性 | Detects human Purine Nucleoside Phosphorylase/PNP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 50 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4860 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Purine Nucleoside Phosphorylase/PNP Met1-Ser289 Accession # P00491 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Purine Nucleoside Phosphorylase (PNP) catalyzes the phophorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. Physiological substrates of PNP include inosine, guanosine, and 2-deoxyguanosine, but not adenosine (1). PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T‑lymphocyte function and neurologic dysfunction (2, 3). PNP is used in assays for the measurement of inorganic phosphate (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Purine Nucleoside Phosphorylase/ PNP by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, MDA‑MB‑231 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, LNCaP human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Purine Nucleoside Phosphorylase/PNP by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 36 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Purine Nucleoside Phosphorylase/PNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6486) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
