| 货号 | AF6479-SP |
| 别名 | brain abundant, membrane attached signal protein 1,22 kDa neuronal tissue-enriched acidic protein; brain acid-soluble protein 1; CAP23; CAP-23; CAP23brain acid soluble protein 1; NAP22; NAP-22; NAP22MGC8555; Neuronal axonal membrane protein NAP-22; neuronal tissue-enriched acidic protein | 全称 | Brain Acid Soluble Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human BASP1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10409 (Human); 70350 (Mouse); 64160 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BASP1 Gly2-Ala45 Accession # P80723 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | BASP1 (Brain acid soluble protein 1; also NAP-22) is a transcriptional cosuppressor that belongs to the BASP1 family of proteins. Although its predicted MW is 23 kDa, BASP1 will show multiple bands in SDS-Page. First, BASP1 forms oligomers in SDS, ranging in size from 30-150 kDa. Second, varying concentrations of SDS will change the apparent BASP1 MW from 56 kDa in 8% SDS-Page to 41 kDa in 13% SDS-Page. At least two forms of BASP1 apparently exist in human, one that is 48-52 kDa, and another that is 32-40 kDa in size. They are not functionally equivalent. BASP1 can be SUMOylated that add 20 kDa in apparent MW. There are also N-terminal fragments termed BIRPs that run from 30-50 kDa in 12% acidic SDS-Page. BASP1 is expressed in neurons, renal podocytes and spermatids. It binds to the cytoplasmic side of the plasmalemma via its myristroylate adduct, and undergoes nuclear translocation when SUMOylated. Human BASP1 is 227 amino acids (aa) in length. It contains a myristoylation site at Gly2, SUMOylation sites at Lys79 and 84, at least eight utilized phosphorylation sites, and an NLS. One splice variant shows a deletion of aa 88-141. Over aa 1-45, human BASP1 shares 96% and 91% aa identity with mouse and rat BASP1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human BASP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BASP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6479) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BASP1 at approximately 60 kDa, possibly a higher molecular weight isoform or a product of SUMOyation. Lower molecular weight isoforms were also observed. This experiment was conducted under reducing conditions on a 4-20% SDS-PAGE gel and using Immunoblot Buffer Group 1. |
