| 货号 | AF6477-SP |
| 别名 | CT31; CT31FLJ10538; EC 1.14.11; EC 1.14.11.-; homolog 1A; Jarid1B; JARID1BFLJ16281; Jumonji, AT rich interactive domain 1B (RBP2-like); jumonji, AT rich interactive domain 1B; KDM5B; lysine (K)-specific demethylase 5B; PLU-1; PLU-1FLJ12459; PLU1FLJ23670; PUT1; RBBP2H1; RBBP2H1AFLJ12491; RBP2-H1; Retinoblastoma-binding protein 2 homolog 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human KDM5B in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10765 (Human); 75605 (Mouse); 304809 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human KDM5B Leu819-Arg918 Accession # Q9UGL1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | KDM5B (also JARID1B, RBP2-H1 and PLU-1) is a 170-200 kDa member of the JARID1 histone demethylase family of proteins. It is a transcriptional repressor that is highly expressed in Sertoli cells and mammary epithelium during pregnancy, and interacts with BF-1 and PAX9. KDM5B is reported to demethylate tri- and di-methylated Lys4 on histone H3. It has no activity on Lys9 or 27. Human KDM5B is 1544 amino acids (aa) in length. It contains a jumonji N domain (aa 32-73), an ARID DNA binding region (aa 97-187), a PHD domain (aa 309-359), a jumonji C domain (aa 486-602), one C5HC2 motif (aa 692-745), and two consecutive PHD domains (aa 1176-1538). There are three utilized phosphorylation sites at Ser1383, 1384 and 1405.There are three potential splice variants. Two show a premature truncation after Pro1433 coupled to an alternative start site at either Met396 or Met159. A third contains a 36 aa insertion after Glu237. Over aa 819-918, human KDM5B shares 96% aa identity with mouse KDM5B. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Lysine (K)-specific Demethylase 5B/KDM5B by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 5B/KDM5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6477) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 5B/KDM5B at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Lysine (K)-specific Demethylase 5B/KDM5B in BT‑20 Human Cell Line. Lysine (K)-specific Demethylase 5B/KDM5B was detected in immersion fixed BT‑20 human breast cancer cell line using Human Lysine (K)-specific Demethylase 5B/KDM5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6477) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
