| 货号 | AF6470-SP |
| 别名 | empty spiracles homeobox 2; empty spiracles homolog 2 (Drosophila); Empty spiracles homolog 2; Empty spiracles-like protein 2; homeobox protein EMX2 | 全称 | Empty Spiracles Homeobox 2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human EMX2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2018 (Human); 13797 (Mouse); 499380 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human EMX2 Asn49-Ser143 Accession # Q04743 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | EMX-2 (also Empty spiracles homolog 2) is a 30-33 kDa member of the EMX homeobox family of molecules. It is expressed in embryonic neural stem and progenitor cells, and promotes their commitment to a neuronal phenotype. In a more global sense, it also promotes the formation of networks that favor visual sensation. In the adult, EMX-2 is found in neural stem cells where it acts to maintain a pool of available stem cells. Human EMX-2 is 252 amino acids (aa) in length. It contains one poly-Ala region (aa 54-59) and a DNA-binding homeobox domain (aa 154-213). There is one potential splice variant that shows a 33 aa substitution for aa 137-252. Over aa 59-143, human EMX-2 shares 98% aa identity with mouse EMX-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse EMX2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Human EMX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6470) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMX2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
