| 货号 | AF6429-SP |
| 别名 | MIG12; migration-inducing gene 12; migration-inducing protein 12; PTMB10; TB10; THYB10; thymosin beta 10; thymosin beta-10; TMSB10 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Thymosin beta 10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Thymosin beta 4 and rhThymosin beta 16 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9168 (Human); 19240 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Thymosin beta 10 Ala2-Ser44 Accession # P63313 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Thymosin beta 10 (T beta 10) is a 6.5 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 5.0-7.0. Multiple cell types produce T beta 10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. T beta 10 is principally an intracellular molecule. It forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis. Mature human T beta 10 is 43 aa in length (aa 2-44) (SwissProt #:P63313). It contains an actin-binding site (aa 18-23), an N-terminal acetylated alanine, four acetylated lysines (#4; 15; 26; 39), and one phosphorylation site at Thr23. Mature T beta 10 is identical to mouse T beta 10 in aa sequence, and it shares 74% aa sequence identity with its human family member T beta 4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Thymosin beta 10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 10 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
