| 货号 | AF6404-SP |
| 别名 | DD5; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase, HECT domain-containing 1; EC 6.3.2; EC 6.3.2.-; EDD; EDD1; EDD1E3 ubiquitin-protein ligase UBR5; EDDE3 identified by differential display; hHYD; HYD; HYDFLJ11310; Hyperplastic discs protein homolog; KIAA0896MGC57263; progestin induced protein; Progestin-induced protein; ubiquitin protein ligase E3 component n-recognin 5; ubiquitin-protein ligase | 全称 | Ubiquitin Protein Ligase E3 Component N-recognin 5 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat UBR5 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51366 (Human); 70790 (Mouse); 117060 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human UBR5 Met1-Asp227 Accession # O95071 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280‑300 kDa member of the HECT E3 family of ubiquitin (Ub) protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799 amino acids (aa) in length and contains a Ub-associated domain (aa 180‑215), two NLSs (aa 502‑517 and 630‑635), one UBR-type zinc finger region (aa 1177‑1244), a PABC region (aa 2377‑2454) and a HECT domain with a Ub-binding Cys residue (aa 2462‑2799). Over aa 1‑227, human UBR5 shares 98% aa identity with mouse UBR5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human, Mouse, and Rat UBR5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and rat ovary tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UBR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBR5 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
