| 货号 | AF6399-SP |
| 别名 | D16S432E; EC 1.1.1; EC 1.1.1.-; FOR; FORHHCMA56; FRA16D; Fragile site FRA16D oxidoreductase; SDR41C1; short chain dehydrogenase/reductase family 41C, member 1; WOX1; WOX1PRO0128; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX | 全称 | WW Domain Containing Oxidoreductase |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat WWOX in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51741 (Human); 80707 (Mouse); 292041 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human WWOX Asn36-Val126 Accession # Q9NZC7 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | WWOX (WW domain-containing oxidoreductase; also WOX1 and FOR) is a 46 kDa cytoplasmic member of the short-chain dehydrogenases/reductases (or SDR) family of molecules. It is expressed in divergent cell types such as breast epithelium, keratinocytes, osteoblasts, and osteoclasts. WWOX has multiple effects, one of which is that of tumor suppressor. Here, WWOX is likely activated by either TGF-beta binding to surface hyaluronoglucosaminidase 2, or C1q binding to C1qR, which, in both cases, initiates cell apoptosis. Human WWOX is 414 amino acids (aa) in length and contains two WW (TrpTrp) domains (aa 16‑90) with an intervening NLS (aa 50‑55), plus an SDR region that contains a mitochondrial targeting sequence (aa 121‑330). There are multiple phosphorylation sites and multiple splice variants that impact intracellular localization. One shows a deletion of aa 173‑352, a second shows a Lys substitution for aa 36‑414, a third contains a 17 aa substitution for |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat WWOX by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat WWOX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6399) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for WWOX at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
