| 货号 | AF6396-SP |
| 别名 | EDG4; EDG-4; EDG4FLJ93869; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor4,LPA receptor EDG4; G protein-coupled receptor; LPA2; LPA-2; LPA2LPA receptor 2; LPAR2; lysophosphatidic acid receptor 2; lysophosphatidic acid receptor EDG4; Lysophosphatidic acid receptor Edg-4 | 全称 | Lysophosphatidic Acid Receptor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9170 (Human); 53978 (Mouse); 498609 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LPAR2 Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279 Accession # Q9HBW0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LPAR2 (Lysophosphatidic acid receptor 2; also LPA2 and EDG4) is a 39 kDa member of the GPCR1 family of proteins. It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes. LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets. Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor. LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding. Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0). It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail. There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endotheliumdifferentiationgene 4). This shows a 35 aa substitution for aa #348-351. Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LPAR2 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
