Human EGLN2/PHD1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human EGLN2/PHD1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6394-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6394-SP
别名	DKFZp434E026; EC 1.14.11; EC 1.14.11.-; egl nine homolog 2 (C. elegans); egl nine homolog 2; EIT6; Estrogen-induced tag 6; FLJ95603; HIFPH1 EGL nine (C.elegans) homolog 2; HIFPH1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; PHD1; Prolyl hydroxylase domain-containing protein 1
全称	Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human EGLN2/PHD1 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	112398 (Human); 112406 (Mouse); 308457 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1? and causes cancer cell death under hypoxia Authors: Eun-Taex Oh Sci Rep, 2016;6(0):27382. Species: Human Sample Type: Cell Lysate Application: WB Ginsenoside 20(S)-Rg3 targets HIF-1alpha to block hypoxia-induced epithelial-mesenchymal transition in ovarian cancer cells. Authors: Liu T, Zhao L, Zhang Y, Chen W, Liu D, Hou H, Ding L, Li X PLoS ONE, 2014;9(9):e103887. Species: Human Sample Type: cell lyates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human EGLN2/PHD1 Asp2-Thr407 Accession # Q96KS0
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	EGLN2 (EGL/EGg Laying-Nine #2; also known as PHD1 and HPH3) is a 43 kDa member of the EglN family of proteins. It is ubiquitously expressed and found principally in the nucleus. EGLN2 hydroxylates proline on HIF-1 alpha. HIF-1 is an alpha / beta heterodimeric transcriptional activator that upregulates genes involved in mitigating the effects of hypoxia. Normally, and in the presence of abundant oxygen, the HIF-1 alpha -chain is hydroxylated by PHD family members, which results in its ubiquitylation and degradation. Under low oxygen tension, EGLN2 activity is decreased, the HIF-1 alpha subunit is retained, and HIF-1 activates genes. Human EGLN2 is 407 amino acids (aa) in length (SwissProt #:Q96KS0). It contains one iron 2-oxoglutarate (Fe2OG) dioxygenase domain (aa 278-376) plus an iron-binding (His297 and His358), and a 2-oxoglutarate-binding (Arg367) site. There is one alternative start site at Met34 that generates a 40 kDa isoform. In addition, there is another potential splice form that shows a 16 aa substitution for aa 1-281. Full-length human EGLN2 shares 91% aa sequence identity with mouse EGLN2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Additional Reduction/Oxidation (Redox) Enzymes HIF Transcription Factors Redox Enzymes

参考图片

Detection of Human EGLN2/PHD1 by Western Blot. Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human EGLN2/PHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6394) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EGLN2/PHD1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.