Human Secretin R Affinity Purified Polyclonal Ab (25 UG)
货号：
AF6387-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF6387-SP |
| 别名 | pancreatic secretin receptor; SCTR; SCT-R; secretin receptor; SR |
全称 | Secretin Receptor | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human Secretin R delta 3/4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6344 (Human); 81779 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Secretin R delta 3/4
Splice variant (1-3) |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SCTR (Secretin receptor) is a 51-62 kDa member of the G-protein coupled receptor 2 family. It is found on alveolar epithelium, bile duct epithelium, pancreatic exocrine duct epithelium, and stomach plus duodenal mucosal epithelium. Mature human SCTR is a 418 amino acid (aa) 7-transmembrane glycoprotein (aa 23-440). It contains a 121 aa N-terminal extracellular region (aa 23-143) plus a 48 aa C-terminal cytoplasmic domain (aa 393-440). There is at least one splice variant that shows a deletion of aa 66-101. SCTR forms homodimers and homooligomers, and heterodimerizes with almost all family B GPCRs (VPAC1; VPAC2; GLP1R; GHRHR; etc.). Splice form of human Secretin Receptor with deletion of exons 3 and 4 was found expressed in pancreatic adenocarcinomas and cholangiocellular carcinomas, but not in gastrinomas or nonneoplastic pancreas or liver specimens.The deletion in this splice form is predicted to lead to a frame-shift, early truncation, and total absence of transmembrane segments in the receptor protein, whereas the leader sequence responsible for trafficking of the receptor to the cell membrane is preserved. This encoded a 111-residue peptide with its first 43 residues identical to wild-type receptor; but, subsequent to a shift in coding frame and early truncation, the next 68 residues were unique in the transcriptome/proteome. Our antibody was raised against this unique 68aa sequence resulting from putative frame-shift. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Hayes, G.M. et al. (2007) Gastroenterology 133:853.
- Korner, M. et al. (2005) Am. J. Pathol. 167:959.
- Korner, M. et al. (2006) J. Hepatol. 45:825.
|
参考图片Detection of Human Secretin R delta 3/4 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human Secretin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6387) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Secretin R delta 3/4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
