| 货号 | AF6369-SP |
| 别名 | 11 zinc finger transcriptional repressor; 11-zinc finger protein; 11-Zinc Finger Protein2; CCCTC-binding factor (zinc finger protein); CCCTC-binding Factor; CTCFL paralog; transcriptional repressor CTCF | 全称 | CCCTC-binding Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CTCF in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10664 (Human); 13018 (Mouse); 83726 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CTCF Met1-Ile154 Accession # P49711 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CTCF (CCCTC-binding factor) is a 130‑140 kDa member of the CTCF Zn-finger protein family. Although its predicted MW is 83 kDa, it runs anomalously slow in SDS‑PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 amino acids (aa) in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa 266‑577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of the standard start site, and another at Met570. A third splice variant shows a 62 aa substitution for aa 349‑727. Over aa 1‑154, human CTCF shares 99% aa identity with mouse CTCF. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CTCF by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CTCF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6369) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTCF at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
