| 货号 | AF6365-SP |
| 别名 | C1orf40; chromosome 1 open reading frame 40; FLJ18233; hShisa4; MGC129812; MGC129813; protein shisa-4; shisa homolog 4 (Xenopus laevis); Shisa4; Shisa-4; TMEM58; Transmembrane protein 58MGC102893 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse Shisa‑4 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 149345 (Human); 77552 (Mouse); 360848 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Shisa‑4 Glu29-Thr85 Accession # Q96DD7 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Shisa-4 (Shisa is a form of Japanese sculpture that involves faces/heads; also known as C1orf40, PRMP and TMEM58) is a 26‑32 kDa member of the Shisa-4 subfamily, Shisa family of proteins. It may be found associated with the ER of embryonic limb bud cells, and based on other Shisa proteins, perhaps antagonizes either Wnt or FGF signaling. Shisa-4 is also described as a plasma membrane protein on oligodendroglia that links the ECM to the actin cytoskeleton. Mature human Shisa-4 is a 170 amino acid (aa) type I transmembrane protein. It contains a 60 aa extracellular domain (aa 28‑87) plus an 89 aa cytoplasmic region that possesses a Pro-rich segment (aa 131‑195). Over aa 29‑85, human Shisa-4 shares 96% aa identity with mouse Shisa-4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Shisa‑4 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Shisa‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shisa‑4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
