货号 | AF6355-SP |
别名 | beta-1,3-N-acetylglucosaminyltransferase manic fringe; EC 2.4.1.222; manic fringe (Drosophila) homolog; manic fringe homolog (Drosophila); MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase | 全称 | Manic Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
目标/特异性 | Detects human MFNG in direct ELISAs and Western blots. |
使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4242 (Human) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human MFNG Gly37-Arg321 Accession # O00587 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
背景 | MFNG (Manic fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 52 - 55 kDa member of the glucosyltransferase 31 family. It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling. MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons. Human MFNG is a 321 amino acid (aa) type II transmembrane protein. It contains a short 7 aa cytoplasmic region, plus a 294 aa luminal domain (aa 28-321). There are two potential splice variants, one that shows a 15 aa substitution for aa 104-321, and another that contains a three aa substitution for aa 86-102. Over aa 37-321, human MFNG shares 85% aa identity with mouse MFNG. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human MFNG by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human MFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MFNG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |