| 货号 | AF6347-SP |
| 别名 | insulin receptor substrate 2; IRS-2 | 全称 | Insulin Receptor Substrate 2 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human IRS2 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8660 (Human); 384783 (Mouse); 29376 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IRS2 Pro1035-Ile1136 Accession # Q9Y4H2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules. It is the primary IRS protein found in hematopoietic cells. Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding. This event allows for its subsequent association with PI-3 kinase. Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa. It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075). IRS2 is phosphorylated on multiple Ser and Tyr residues. There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338. Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human IRS2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human IRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6347) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRS2 at approximately 170-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
