| 货号 | AF6346-SP |
| 别名 | COL13A1; collagen alpha-1(XIII) chain; collagen, type XIII, alpha 1; FLJ42485 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Collagen XIII in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse COL13A1 and less than 1% cross-reactivity with recombinant human (rh) COL23A1 and rhCOL25A1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1305 (Human); 12817 (Mouse); 499431 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII Glu109-Leu668 Accession # NP_542992 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the integrin alpha 1 subunit, nidogen-2, and fibronectin. Human COL13A1 is 717 amino acids (aa) in length (SwissProt #:Q5TAT6). It has an N‑terminal 44 amino acid cytoplasmic region plus a 656 amino acid extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, 217-269, 442-463, and 700-717) interspersed among three collagenous (COL) domains (aa 122-216, 270-441, and 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, 551-565, and 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF Membrane was probed with 1 µg/mL of Human Collagen XIII alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Two specific bands were detected for Collagen XIII a1 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
