Human CD40/TNFRSF5 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CD40/TNFRSF5 Affinity Purified Polyclonal Ab (25 UG)

货号： AF632-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF632-SP
别名	B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
反应种属	Human
应用	Western Blot(2 µg/mL)
目标/特异性	Detects human CD40 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD40 and recombinant rat CD40 is observed, and less 1% cross-reactivity with recombinant human (rh) TNF RI, rhsTNF RII, rhCD40 Ligand, rh4-1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhNGF R, rhRANK and rhRELT is observed.
使用方法	Western Blot: 2 µg/mL Agonist Activity: Measured in a cell proliferation assay using B cell enriched human peripheral blood lymphocytes in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED₅₀ for this effect is typically 3-12 ng/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	958 (Human); 21939 (Mouse); 171369 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression, purification and biological characterization of the extracellular domain of CD40 from Pichia pastoris. Authors: Zhan Y, Wei Y, Chen P, Zhang H, Liu D, Zhang J, Liu R, Chen R, Zhang J, Mo W, Zhang X BMC Biotechnol, 2016;16(1):8. Species: Human Sample Type: Cell Lysates Application: WB CD40.FasL inhibits human T cells: evidence for an auto-inhibitory loop-back mechanism. Authors: Dranitzki-Elhalel M, Huang JH, Sasson M, Rachmilewitz J, Parnas M, Tykocinski ML Int. Immunol., 2007;19(4):355-63. Species: Human Sample Type: Cell Lysates Application: WB Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production. Authors: Nemoto E, Tada H, Shimauchi H J. Leukoc. Biol., 2002;72(3):538-45. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD40 Glu21-Arg193 Accession # P25942
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured in a cell proliferation assay using B cell enriched human peripheral blood lymphocytes in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED₅₀ for this effect is typically 3-12 ng/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature hCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 62 aa cytoplasmic domain (1). Human and mouse CD40 share 62% aa identity. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by CD40 binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6 - 8).
运输条件	Blue Ice
存放说明	4℃
参考文献	Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569. Armitage, R.J. et al. (1993) J. Immunol. 150:3671. Callard, R.E. et al. (1993) Immunol. Today 14:559. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487. Pullen, S.S. et al. (1999) Biochemistry 38:10168. Faris, M. et al. (1994) J. Exp. Med. 179:1923. Hanissian, S.H. and R.S Geha (1997) Immunity 6:379.

参考图片

Detection of Human CD40/TNFRSF5 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human CD40/TNFRSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF632) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD40/TNFRSF5 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human CD40/TNFRSF5 Antibody Stimulates Cell Proliferation in Human B Cells. Goat Anti-Human CD40/TNFRSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF632) stimulates human B cell proliferation in the presence of Recombinant Human IL‑4 (Catalog # 204-IL) in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED₅₀ for this effect is typically 3-12 ng/mL