| 货号 | AF6328-SP |
| 别名 | folate receptor 4 (delta) homolog (mouse); Folbp3; FR-delta; LOC390243; probable folate receptor delta | 全称 | Folate Receptor 4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human FOLR4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1, rhFOLR2, and rhFOLR3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 390243 (Human); 64931 (Mouse); 690037 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FOLR4 Glu134-Asn210 Accession # NP_001073955 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FOLR4 (Folate receptor 4; also FR4 and folate receptor δ and folate binding protein 3) is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4hiCD25hi expression defines T reg cells, FR4loCD25+ expression pattern identifies effector memory T cells, and a FR4hiCD25- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human FOLR4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
