| 货号 | AF6324-SP |
| 别名 | odz, odd Oz/ten-m homolog 1(Drosophila); ODZ1; ODZ3; Protein Odd Oz/ten-m homolog 1; Ten-1; tenascin M; tenascin M1; Tenascin-M1; teneurin 1; teneurin-1; TEN-M1; TNM; TNM1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Teneurin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Teneurin-3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10178 (Human); 23963 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ODZ1 allows glioblastoma to sustain invasiveness through a Myc-dependent transcriptional upregulation of RhoA | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Teneurin‑1 Met1-Lys317 Accession # AAF04723 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Teneurin-1 (also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons. Teneurin 1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human Teneurin-1 is a 2725 amino acid (aa) type II TM glycoprotein. It contains a 324 aa cytoplasmic region (aa 1-324) that contains an NLS (aa 62‑65), plus a 2380 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 528-796), five NHL repeats, each of which form a beta -propeller (aa 1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. Cleavage at the N-terminus generates an initial 65 kDa membrane spanning fragment, followed by TM cleavage that generates a 45 kDa cytosolic fragment. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2682-2722) termed TCAP-1 that shows bioactivity. One splice variant shows a deletion of aa 1232-1239. Over aa 1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human Teneurin‑1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin‑1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
