| 货号 | AF6319-SP |
| 别名 | FLJ35767; testis expressed 19; testis-expressed sequence 19 protein | 全称 | Testis Expressed 19 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(10 µg/mL) |
| 目标/特异性 | Detects human TEX19 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TEX11 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 10 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 400629 (Human); 70956 (Mouse); 691099 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TEX19 Met1-Ser164 Accession # Q8NA77 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TEX19 (testis expressed sequence 19) is an 18 kDa (predicted) protein with restricted cell expression. It is expressed in spermatogonia as well as pluripotent stem cells. TEX19 has been found in both nucleus and cytoplasm. Functionally, TEX19 is proposed to guard against inappropriately activated endogenous retroviruses during meiosis, and thus prevent deleterious mutations. Human TEX19 is 164 amino acids (aa) in length. There is no identifiable structural module within the protein. There are, however, two sequences that are conserved in TEX19 from multiple species. These are an MCP domain between aa 1-58, and a VPTEL domain that spans aa 120-157. Over aa 1-164, human TEX19 shares only 53% aa identity with a highly divergent and substantially larger 42 kDa mouse TEX19.1 protein. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TEX19 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEX19 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
TEX19 in BG01V Human Stem Cells. TEX19 was detected in immersion fixed BG01V human embryonic stem cells using Human TEX19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6319) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
