| 货号 | AF6315-SP |
| 别名 | DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; Dnmt3a; DNMT3A2; EC 2.1.1.37; M.HsaIIIA | 全称 | DNA [Cytosine-5-]-Methyltransferase 3A |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DNMT3A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT1 is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1788 (Human); 13435 (Mouse); 444984 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DNMT3A Ala353-Lys486 Accession # Q9Y6K1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DNMT3A (DNA [cytosine-5]-methyltransferase 3A) is a 130 kDa member of the C5-methlytransferase family of molecules. It is expressed both during development and in virtually all adult tissues except for small intestine. DNMT3A is a nuclear protein that is found associated with heterochromatin. It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer. It also interacts with SETDB1 to form a transcriptional repressor. Human DNMT3A is 912 amino acids (aa) in length. It contains one ProTrpTrpPro domain (aa 292-350) and one ADD-type zinc finger region (aa 494-586). There is one 100 kDa splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin. Other potential splice variants show a 16 aa substitution for aa 151-912, and a 24 aa substitution for aa 1-213. Over aa 353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
DNMT3A in HeLa Human Cell Line. DNMT3A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human DNMT3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6315) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
