| 货号 | AF6309-SP |
| 别名 | C20orf183; chromosome 20 open reading frame 183; DAI; DLM1; DLM-1; DLM1dJ718J7.3; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; Tumor stroma and activated macrophage protein DLM-1; ZBP1 Z-DNA binding protein 1; Z-DNA binding protein 1; Z-DNA-binding protein 1 | 全称 | Zipcode-binding Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ZBP1/DLM-1/DAI in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81030 (Human); 58203 (Mouse); 171091 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZBP1/DLM-1/DAI Ala2-Ile429 Accession # Q9H171 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappa B activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human ZBP1/DLM‑1/DAI by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL) and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZBP1/DLM‑1/DAI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6309) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ZBP1/DLM‑1/DAI at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
