| 货号 | AF6307-SP |
| 别名 | AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18 | 全称 | Anterior Gradient 3 Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human AG‑2 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 155465 (Human); 403205 (Mouse); 298959 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E.coli-derived Recombinant Human AG-3 Ile22-Leu166 Accession # Q8TD06 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | AG3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG3 is presumably secreted and binds to both a-dystroglycan and C4.4a/LYPD3. Mature human AG3 is 145 amino acids (aa) in length (aa 22‑166) (SwissProt #:Q8TD06). Over aa 22-166, human AG3 shares 92% amino acid sequence identity with mouse AG3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human AG‑3 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Human AG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG‑3 at approximately 19kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
AG‑3 in Human Breast. AG‑3 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human AG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
