| 货号 | AF6296-SP |
| 别名 | CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1 | 全称 | Tudor Domain Containing 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse TDRD1 is observed and less than 1% cross-reactivity with recombinant human TDRKH is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56165 (Human); 83561 (Mouse); 292129 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TDRD1 Asn837-Lys968 Accession # Q9BXT4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TDRD1 (Tudor domain containing protein 1; also CT41.1) is a 120-140 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Human TDRD1 is 1180 aa in length. It contains one MYND-type Zn finger region (aa 170-206), and four methyl-binding Tudor domains (aa 312-1048). There are four potential splice variants with predicted molecular weights ranging between 79 kDa and 133 kDa. One contains an alternate start site at Met404, another contains an alternative start site at Met22, accompanied by deletions of aa 554-610 and 775-831, a third shows a 10 aa substitution for Ser1180, while a fourth contains deletions of aa 328-375, 554-610 and 1059-1134. Over aa 837-968, human TDRD1 shares 66% aa identity with mouse TDRD1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TDRD1 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6296) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TDRD1 at approximately 75 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
