Human/Mouse PRDM16/MEL1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse PRDM16/MEL1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6295-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6295-SP
别名	MDS1/EVI1-like gene 1; MEL1; MEL1KIAA1675PFM13MGC166915; PFM13; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1
全称	PR Domain Containing 16
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse and human PRDM16 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	63976 (Human); 70673 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16 Authors: Hanying Ding Nat Commun, 2016;7(0):11533. Species: Mouse Sample Type: Tissue Homogenates Application: WB White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C Nat Cell Biol, 2015;17(1):57-67. Species: Human Sample Type: Cell Lysate Application: WB Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma. Authors: Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D Cell, 2012;150(3):620-32. Species: Mouse Sample Type: Cell Lysates Application: WB MyomiR-133 regulates brown fat differentiation through Prdm16. Authors: Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus Nat Cell Biol, 2012;14(12):1330-5. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse PRDM16 Lys537-Glu688 Accession # A2A935.1
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: PRDM16/MEL1 Mesenchymal Stem Cell Transcription Factors Neural Stem Cell Transcription Factors

参考图片

Detection of Human PRDM16 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PRDM16 in Mouse Embryo. PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.