| 货号 | AF6294-SP |
| 别名 | one cut domain family member 2; HNF-6B; OC2; OC-2; one cut homeobox 2 | 全称 | One Cut Homeobox 2 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ONECUT2/OC-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ONECUT1 is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9480 (Human); 225631 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ONECUT2/OC-2 Gln185-Thr326 Accession # O95948 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ONECUT2/OC-2 in HepG2 Human Cell Line. ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
