| 货号 | AF6290-SP |
| 别名 | LIM homeobox 5; LIM homeobox protein 5; LIM/homeobox protein Lhx5; MGC129689 | 全称 | LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64211 (Human); 16873 (Mouse); 124451 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LHX5 Leu265-Trp402 Accession # Q9H2C1 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Rat LHX5 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LHX5 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LHX5 in Mouse Brain. LHX5 was detected in perfusion fixed frozen sections of adult mouse brain (hippocampus) using Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
