| 货号 | AF6286-SP |
| 别名 | TDRD2; tudor and KH domain containing; tudor and KH domain-containing protein | 全称 | Tudor and KH Domain Containing |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TDRKH in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11022 (Human); 72634 (Mouse); 310652 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TDRKH His206-Leu561 Accession # Q9Y2W6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins. It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells. TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome. Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for non-coding RNA binding. Human TDRKH is 606 aa in length. It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412). There are four potential splice forms. Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606. Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TDRKH by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Human TDRKH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6286) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TDRKH at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
TDRKH in Mouse Testis. TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Human TDRKH Affinity-purified Polyclonal Antibody (Catalog # AF6286) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to germ cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
