| 货号 | AF6277-SP |
| 别名 | HNF-6A; HNF6Ahepatocyte nuclear factor 6, alpha; HNF6hepatocyte nuclear factor 6; HNF-6One cut domain family member 1; OC-1; one cut homeobox 1one cut domain, family member 1; ONECUT1 | 全称 | Hepatocyte Nuclear Factor 6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3175 (Human); 15379 (Mouse); 25231 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human HNF-6/ONECUT1 Gln139-Gly283 Accession # Q9UBC0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human HNF‑6/ONECUT1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF‑6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HNF‑6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
HNF‑6/ONECUT1 in HepG2 Human Cell Line. HNF‑6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF‑6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
