| 货号 | AF6237-SP |
| 别名 | beta-hexosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; EC 3.2.1; EC 3.2.1.52; HEXA; hexosaminidase A (alpha polypeptide); Hexosaminidase subunit A; MGC99608; N-acetyl-beta-glucosaminidase subunit alpha; TSD |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Simple Western(20 µg/mL) |
| 目标/特异性 | Detects human Hexosaminidase A/HEXA in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Simple Western: 20 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3073 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Hexosaminidase A/HEXA Leu23-Thr529 Accession # P06865 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | HEXA (beta-hexosaminidase subunit alpha; also hexaminidase subunit alpha) is a 50-56 kDa glycoprotein member of the glycosyl hydrolase #20 family of enzymes. It is found in the lysosomes of a variety of cell types including fibroblasts, lacrimal acinar cells, and neurons. beta -Hexosaminidase is a multimeric enzyme that cleaves aminoacetylhexosamines from multiple glycosylated molecules. It exists in three noncovalent isozyme forms, among which is an A form that is trimeric and composed of one alpha - and two beta -subunits, a B form that is tetrameric and contains two alpha - and two beta -subunits, and a dimeric S form that contains two alpha -subunits. The alpha - and beta -subunits are products of related but distinct genes. The beta -hexosiminidase A form acts on a wide range of substrates, while the B form activity is biased towards sulfated hexoses. Human HEXA is synthesized as a 529 aa (amino acid) prepro-precursor. It contains a 22 aa signal sequence, a 66 aa prosegment, and a 441 aa mature region that contains a catalytic domain (aa 167-488). The prosegment may undergo additional processing through His90, and this segment (≈ 6 kDa) is known to remain linked to the mature region via a disulfide bond. Notably, circulating HEXA is comprised only of subunit proforms whose MW may run some 10 kDa higher in SDS-PAGE. Over aa 23-529, human HEXA shares 85% and 57% aa sequence identity with mouse HEXA and the human HEXA beta -subunit proprecursor, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Hexosaminidase A/ HEXA by Western Blot. Western blot shows lysate of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexosaminidase A/HEXA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Hexosaminidase A/HEXA by Simple WesternTM. Simple Western lane view shows lysate of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Hexosaminidase A/HEXA at approximately 59 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
