| 货号 | AF6236-SP |
| 别名 | EC 2.8.2.20; TPST-2; tyrosylprotein phosphotransferase 2; Tyrosylprotein Sulfotransferase 2; tyrosylprotein sulfotransferase 2protein-tyrosine sulfotransferase 2; tyrosylprotein sulfotransferase-2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human Tyrosylprotein Sulfotransferase 2/TPST2 in direct ELISAs and Western blots. In direct ELISAs, less than 7% cross-reactivity with recombinant human TPST1 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8459 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recominant human Tyrosylprotein Sulfotransferase 2/TPST2 Gln26-Ser377 (predicted) Accession # O60704 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Tyrosylprotein Sulfotransferase 2 (TPST2) is a 42kDa (predicted) member of a sulfotransferase family. Two human tyrosylproteinsulfotransferases (TPSTs), TPST1 and TPST2 mediate tyrosine O-sulfation of morethan 60 different proteins in the Golgi, transferring sulfate from3-phosphoadenosine 5‑phosphosulfate to tyrosine residues contained inpolypeptides with acidic motifs to form a tyrosine O-sulfate ester. TSPT1 and TSPT2are widely expressed, they show similar tissue distribution and overlappingsubstrate specificity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Tyrosylprotein Sulfotransferase 2/TPST2 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tyrosylprotein Sulfotransferase 2/TPST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6236) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tyrosylprotein Sulfotransferase 2/TPST2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
