| 货号 | AF6216-SP |
| 别名 | MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta | 全称 | Toll-like receptor adaptor molecule 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TRIF/TICAM1 in Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 148022 (Human); 106759 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TRIM38 Negatively Regulates TLR3-Mediated IFN-beta Signaling by Targeting TRIF for Degradation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRIF/TICAM1 Lys29-Ala204 Accession # Q8IUC6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF 3, -4, and -7, as well as NF kappa B and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length and contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 29-204, human TRIF shares 62% aa identity with mouse TRIF. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRIF/TICAM1 by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line and Raji human Burkitts lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
TRIF/TICAM1 in Raji Human Cell Line. TRIF/TICAM1 was detected in immersion fixed Raji human Burkitts lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
