| 货号 | AF6206-SP |
| 别名 | importin subunit alpha-1; importin-alpha-S1; IPOA5; karyopherin alpha 1 (importin alpha 5); Karyopherin subunit alpha-1; KPNA1; NPI-1; NPI-1SRP1importin alpha 5; Nucleoprotein interactor 1; RCH2; RCH2RAG cohort protein 2; recombination activating gene cohort 2; SRP1; SRP1-beta |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Importin alpha 5/KPNA1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3836 (Human); 16646 (Mouse); 288064 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Importin alpha 5/KPNA1 Met1-Val132 Accession # P52294 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Importin subunit alpha-5 (KPNA1, Karyopherin subunit alpha 1; also SRP1 beta, NPI-1 and RAG2) is a 65 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA1 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. Two scenarios are possible. First, NLS‑containing cargo molecules bind to KPNA1, which then binds to importin beta ; alternatively, KPNA1 and importin beta initially form a complex that primes KPNA1 for subsequent cargo binding. In either case, importin beta binds to the nuclear pore, facilitating transport into the nucleus. Human KPNA1 is 538 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 1‑57), ten Armadillo repeats that bind "cargo" (aa 77‑504) and two intervening NLS binding sites. Over aa 1-132, human KPNA1 shares 97% aa identity with mouse KPNA1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Importin alpha 5/KPNA1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Importin alpha 5/KPNA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Importin alpha 5/KPNA1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
