| 货号 | AF6195-SP |
| 别名 | ATP-dependent helicase CHD1; CHD-1; chromodomain helicase DNA binding protein 1; chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; EC 3.6.1; EC 3.6.4.12 | 全称 | Chromodomain Helicase DNA Binding Protein 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse CHD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human CHD5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1105 (Human); 12648 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CHD1 Lys1531-Thr1710 Accession # O14646 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CHD1 (Chromohelicase/ATPase DNA‑binding protein 1) is a 200‑220 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser‑rich regions (aa 1‑70 and 117‑137), two Chromo (chromatin‑organizer‑modifer) domains (aa 272‑364 and 389‑452), a SNF2 family N‑terminal domain (aa 484‑763) and a C‑terminal helicase domain (aa 792‑943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531‑1710, human CHD1 shares 92% aa identity with mouse CHD1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CHD1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse CHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
