| 货号 | AF6157-SP |
| 别名 | hSPRY2; MGC23039; protein sprouty homolog 2; sprouty (Drosophila) homolog 2; sprouty 2; sprouty homolog 2 (Drosophila); spry-2 | 全称 | Sprouty Homolog 2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Simple Western(50 µg/mL) |
| 目标/特异性 | Detects human and mouse SPRY2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 50 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10253 (Human); 24064 (Mouse); 306141 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SPRY2 Met1-Ala175 Accession # O43597 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKCδ and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse SPRY2 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, PC‑3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SPRY2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human and Mouse SPRY2 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and SH‑SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
