| 货号 | AF6139-SP |
| 别名 | HRASLS4; PLA1/2-3; RAR-responsive protein TIG3; retinoic acid receptor responder (tazarotene induced) 3; retinoic acid receptor responder protein 3; retinoic acid-inducible gene 1; Retinoid-inducible gene 1 protein; RIG1; Tazarotene-induced gene 3 protein; TIG3; TIG3MGC8906 | 全称 | Retinoic Acid Receptor Responder [Tazarotene-induced] 3 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human RARRES3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) RARRES1 and rhLatexin is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5920 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RARRES3 Asp12-Lys134 Accession # Q9UL19 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RARRES3 (Retinoic acid receptor responder 3; also Tig3) is an 18-20 kDa member of the lecithin retinol acyltransferase family of molecules. It is widely expressed, particularly in keratinocytes, and is considered to have multiple functions. These include phospholipase A activity and an ability to both activate type I transglutaminase, and inhibit Ras activation Human RARRES3 is 164 amino acids (aa) in length. It contains a catalytic residue at Cys113, and a hydrophobic membrane-anchoring domain between aa 134-164. Within the cell, RARRES3 appears to be equally distributed between membrane and soluble fractions. RARRES3 is also reported to form higher order multimers. One potential isoform exists that shows a 26 aa substitution for aa 131-164. No meaningful structural ortholog of RARRES3 exists in rodent. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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RARRES3 in Raji Human Cell Line. RARRES3 was detected in immersion fixed Raji human Burkitts lymphoma cell line using Human RARRES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
