| 货号 | AF6115-SP |
| 别名 | DKFZp434K2235; FLJ90319; Histone deacetylase complex subunit Sin3a; KIAA0700; paired amphipathic helix protein Sin3a; SIN3 homolog A, transcription regulator (yeast); SIN3 homolog A, transcriptional regulator (yeast); Transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; transcriptional regulator, SIN3A | 全称 | SIN3 Homolog A |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse SIN3A in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25942 (Human); 20466 (Mouse); 363067 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SIN3A Tyr1187-Pro1273 Accession # Q96ST3 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SIN3A (Switch insensitive-3 A) is a nuclear, 140‑150 kDa member of the SIN3 family of co-repressors. SIN3A plays a key role in gene repression. Normally, genes are not expressed when chromatin is "closed", or condensed, precluding transcriptional activators from assembling on gene promoters. This state is achieved through histone deacetylation and DNA methylation, and SIN3A serves as a scaffold for multiple proteins, including deacetylases (HDAC1 and 2). Human SIN3A is 1273 amino acids (aa) in length. It contains three PAH (paired amphipathic helix) repeats (aa 141‑187, 322‑381 and 477‑523) and multiple phosphoserines and acetylated lysines. There are three potential splice variants. The first shows a 33 aa substtution for aa 124‑1273, while the second and third show eight aa substitutions for aa 337‑352, coupled to either a deletion of aa 1009‑1066, or a two aa substitution for aa 1008‑1065. Over aa 1187‑1273, human and mouse SIN3A show 100% identity in aa sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SIN3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SIN3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6115) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SIN3A at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
