| 货号 | AF6099-SP |
| 别名 | DAGK5; DAGK6DAG kinase zeta; DGKZ; DGK-ZETA; diacylglycerol kinase zeta; diacylglycerol kinase, zeta 104kDa; diacylglycerol kinase, zeta; Diglyceride kinase zeta; EC 2.7.1.107; hDGKzeta | 全称 | Diacylglycerol Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human DGK‑ zeta in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8525 (Human); 104418 (Mouse); 81821 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DGK‑ zeta Gln800-Val945 Accession # Q13574 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DGK-zeta (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130 kDa member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-zeta converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-zeta -generated PA activates PAK1, leading to increased cell motility. Human DGK-zeta is 1117 amino acids (aa) in length and contains two DAG-type Zn-finger regions (aa 287‑419), a MARCKS homology domain (aa 448‑462) and a catalytic domain (aa 486‑607). In addition to the 130 kDa protein, there are several splice variants that run about 114 kDa in SDS-PAGE. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human DGK‑ zeta by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DGK‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6099) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for DGK‑ zeta at approximately 124 & 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
