| 货号 | AF6096-SP |
| 别名 | 1110020L01Rik; Amplaxin; cortactin; CTTN; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate); EMS1; EMS1amplaxin; FLJ34459; Oncogene EMS1; src substrate cortactin |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and rat Cortactin in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2017 (Human); 13043 (Mouse); 60465 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cortactin Met1-Gly85 Accession # Q14247 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cortactin (also EMS1 and Amplaxin) is an 80‑85 kDa multidomain scaffold-associated protein. It is an actin-binding cytoplasmic protein found at intercellular junctions and near lamellipodia. It is also found in tumor invadopodia, a somewhat analogous structure to lamellipodia. Here, Cortactin appears to coordinate actin assembly with MMP secretion, thus facilitating tumor invasiveness. Human Cortactin is 550 amino acids (aa) in length and contains six-plus Cortactin regions (aa 80‑324) that regulate actin polymerization, and one SH3 domain (aa 496‑548) that binds to N-WASP. Cortactin is highly phosphorylated on Tyr and Ser/Thr, and undergoes acetylation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat Cortactin by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, MDA‑MB‑453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Cortactin at approximately 80‑85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
