| 货号 | AF6090-SP |
| 别名 | JARID2; JMJ; JMJjumonji-like protein; jumonji (mouse) homolog; jumonji homolog; jumonji, AT rich interactive domain 2; Jumonji/ARID domain-containing protein 2; protein Jumonji |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human and mouse Jumonji/JARID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human JARID2B is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3720 (Human); 16468 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Jumonji/JARID2 Met1-Thr159 Accession # Q92833 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Jumonji/JARID2 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
