| 货号 | AF6068-SP |
| 别名 | AG-2; AG2member 17; AGR2; anterior gradient 2 homolog (Xenopus laevis); anterior gradient homolog 2 (Xenopus laevis); GOB-4; hAG-2; HPC8; PDIA17; secreted cement gland homolog | 全称 | Anterior Gradient 2 Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human AG‑2 in direct ELISA and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with rhAG-3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10551 (Human); 23795 (Mouse); 298961 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AG‑2 Arg21-Leu175 Accession # O95994 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18‑21 kDa member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155 amino acids (aa) in length (aa 21‑175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20 aa upstream of the standard start site. Over aa 21‑175, human AG-2 shares 94% aa identity with mouse AG-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human AG-2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and human small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG-2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
AG‑2 in Human Breast Cancer Tissue. AG‑2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human AG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6068) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
