| 货号 | AF6048-SP |
| 别名 | EA6; EA6FLJ25094; EAAT1GLAST-1; excitatory amino acid transporter 1; GLAST-1; GLASTGLAST1; SLC1A3; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3 | 全称 | Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human EAAT1/GLAST‑1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6507 (Human); 20512 (Mouse); 29483 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human EAAT1/GLAST‑1 His146-Ser237 Accession # P43003 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GLAST-1 (Sodium dependent GLu/ASp Transporter 1; also SLC1A3 and EAAT1) is a 60‑62 kDa member of the SDF symporter family of molecules. It is expressed by glia, fibroblasts, and select neuron cell types such as hippocampal neurons. EAAT1 is known to transport L‑glutamate into glia, thus removing glutamate from synaptic areas where it either acts too long, or becomes toxic at elevated concentration. Its action is dependent on its ability to cotransport sodium. Human EAAT1 is a 542 amino acid (aa), 8-transmembrane variably glycosylated protein that contains N- and C-terminal cytoplasmic domains. Homodimers are reported to exist, but only when EAAT1 shows glycosylation. There are at least two potential isoform variants. One shows an Arg substitution for aa 430‑475, while a second shows a four aa substitution for aa 62‑542. Over an extracellular loop that encompasses aa 146‑237, human EAAT1 shares 92% aa identity with mouse EAAT1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human EAAT1/GLAST‑1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human EAAT1/GLAST‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EAAT1/GLAST‑1 at approximately 60 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
EAAT1/GLAST‑1 in Human Brain. EAAT1/GLAST‑1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Sheep Anti-Human EAAT1/GLAST‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6048) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes and presynaptic profiles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
