Human Carboxypeptidase B2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Carboxypeptidase B2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6036-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6036-SP
别名	carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 3.4.17.20; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CPA1, rhCPB1 and recombinant mouse CPB1 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1361 (Human); 56373 (Mouse); 113936 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2 Phe23-Val423 (Ala169Thr) Accession # Q96IY4
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes. It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation. CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa). Active CPB2 removes C-terminal Lys residues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization. Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length. It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminal Lys or Arg residues. There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423. Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Coagulation Metalloproteases and Regulators

参考图片

Detection of Human Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human platelets. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase B2/CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxypeptidase B2/CPB2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Carboxypeptidase B2/CPB2 in Human Liver and Liver Cancer Tissue. Carboxypeptidase B2/CPB2 was detected in immersion fixed paraffin-embedded sections of normal human liver (left panel) and human liver cancer tissue (right panel) using Sheep Anti-Human Carboxypeptidase B2/
CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.