| 货号 | AF6026-SP |
| 别名 | FA; FA1; FAA; FAAMGC75158; FACAFanconi anemia group A protein; FAH; FA-H; FANCA; FANCH; Fanconi anemia, complementation group A; Fanconi anemia, complementation group H; Fanconi anemia, type 1; Protein FACA | 全称 | Fanconi Anemia, Complementation Group A |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human FACA/FANCA in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse FANCA, recombinant human (rh) FANCD2, rhFANCE, rhFANCF, rhFANCG, and rhFANCI is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2175 (Human); 14087 (Mouse); 361435 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FACA/FANCA Tyr35-Arg142 Accession # O15360 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FACA (Fanconi anemia group A) is a 160‑165 kDa protein that belongs to Fanconi anemia protein group I. FACA is widely expressed, activated by phosphorylation, partners with FANCG, and participates in the formation of an eight subunit Fanconi anemia core complex that ubiquitinates FANCD2 and FANCI. Ultimately, this contributes to the reactivation of stalled DNA repair replication forks. Human FACA is 1455 amino acids (aa) in length. It contains one NLS (aa 18‑34) and four phosphorylation sites at Ser849, 850, 858 and 1449. There are four potential splice variants. One shows a premature truncation after Cys297, a second contains a six aa substitution for aa 297‑1455, a third shows a deletion of aa 143‑174 accompanied by truncation after Cys297, and a fourth exhibits an alternative start site at Met528, accompanied by a 35 aa substitution for aa 1390‑1455. Over aa 35‑142, human FACA shares 61% aa identity with mouse FACA. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FACA/FANCA by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FACA/FANCA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6026) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FACA/FANCA at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
