Human Cyr61/CCN1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Cyr61/CCN1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6009-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6009-SP
别名	CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1
全称	Cysteine-Rich, Angiogenic Inducer 61
反应种属	Human
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Detects human Cyr61/CCN1 in Western blots.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3491 (Human); 16007 (Mouse); 83476 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Cyr61/CCN1 Ala22-Asp381 Accession # O00622
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cysteine-rich angiogenic inducer 61 (Cyr61), also known as CCN1, is a 40‑45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4‑6). Plasmin cleavage of ECM‑bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8‑12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337. Jay, P. et al. (1997) Oncogene 14:1753. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98. Chen, N. et al. (2000) J. Biol. Chem. 275:24953. Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248. Schutze, N. et al. (2007) BMC Cell Biol. 8:45. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705. OKelly, J. et al. (2008) Int. J. Oncol. 33:59. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696. Xie, D. et al. (2004) Cancer Res. 64:1987. Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594. Monnier, Y. et al. (2008) Cancer Res. 68:7323. Tong, X. et al. (2001) J. Biol. Chem. 276:47709. Chien, W. et al. (2004) J. Biol. Chem. 279:53087. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014. Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.

参考图片

Detection of Human Cyr61/CCN1 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and SK‑BR‑3 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human Cyr61/CCN1 by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 52 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.