| 货号 | AF6007-SP |
| 别名 | EC 3.4.19.12; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCH-L1 | 全称 | Ubiquitin C-terminal Hydrolase L1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat UCH-L1/PGP9.5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human UCH-L3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7345 (Human); 22223 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human UCH-L1/PGP9.5 Gln2-Ala223 Accession # P09936 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Deubiquitination is a critical regulatory process in the ubiquitin-proteasome pathway (1). Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine proteases that catalyze the hydrolysis of a peptide bond at the C-terminal glycine of ubiquitin. Members of the UCH family have been implicated in a number of human diseases, including neurodegenerative diseases and cancers (2). Mutations of the UCH-L1 gene and alterations of the protein activity have been found to be associated with several neurodegenerative disorders, including Parkinson’s, Huntington’s and Alzheimer’s diseases (3). It is also implicated in cancer tumorigenesis, including lung, breast, liver, kidney, colorectal and ovarian cancers (4‑8). UCH-L1 is thought to be a tumor suppressor and biomarker for hepatocellular carcinoma and other digestive tumors. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, PC‑12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat UCH-L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human and Mouse UCH‑L1 by Simple WesternTM. Simple Western lane view shows lysates of A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UCH‑L1/ PGP9.5 at approximately 30 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat UCH‑L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
