货号 | AF6005-SP |
别名 | MEAI; MEN1; Menin; multiple endocrine neoplasia I; multiple | 全称 | Multiple Endocrine Neoplasia I |
反应种属 | Human |
应用 | Western Blot(2 µg/mL) |
目标/特异性 | Detects human Menin in Western blots. |
使用方法 | Western Blot: 2 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4221 (Human); 17283 (Mouse); 29417 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human Menin aa 555-615 Accession # O00255 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human Menin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Menin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Menin at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |